Sentence examples for sequencing template from inspiring English sources

This protocol was used to obtain sequencing template from reoviruses of three different vertebrate classes.

By amplifying the whole blood in a single tube with one-step process to get the sequencing template, alcohol dehydrogenase (ADH) gene polymorphisms of 24 clinical blood samples were successfully detected, and the results could served as a guide for individualized medication clinically.

E. coli cultures were amplified by rolling circle amplification (RCA) using the Templiphi™ DNA Sequencing Template Amplification Kit (GE Healthcare, Piscataway, NJ).

However, when using PCR products obtained by short-range PCR amplification as Sanger sequencing template, electropherograms showed clearly heterozygous peaks for these same SNPs.

When the Excess Primer Strand is used as the sequencing template, there is a very low level of background signal in the chromatogram.

Plasmid DNA preparation was performed with the TempliPhi DNA sequencing template amplification kit (GE Healthcare -Bio-Sciences) or Splasmidepreparationreparation kits (Agencourt Bioscience).

The B-F-CHPV-U1 primer used for universal probe amplification was biotinylated, and the leading strand could therefore be used as a sequencing template.

A pair of primers, ORF100-INDE were used to amplify the sequencing templates.

Fragments (200 ± 25 bp) were then separated by agarose gel electrophoresis and selected for PCR amplification as sequencing templates.

The technique allows amplification of nucleic acid sequences both for the purposes of disease and pathogen detection and also for the preparation of hybridization probes and sequencing templates.

Preparation of sequencing templates was carried out following standard protocols, employing FIREPol polymerase (Solis BioDyne).