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In a process known as assembly, computer programs were then used to stitch the sequences back together to reconstruct the original DNA sequencing target.
Due to the weaknesses of target sequencing and metagenomic sequencing, single-cell sequencing is becoming a powerful complementary approach, which aims at sequencing target bacteria at single cell levels.
To ensure that the project did not overrun or fail to achieve its sequencing target, it was crucial that strict deadlines for submitting samples to the project were adhered to by participating collaborators so that delays to the sequencing pipeline at the Wellcome Trust Sanger Institute (WTSI) were minimized.
This was possible due to cytogenetic analysis of rice showing the diminutive centromere 8. Interestingly, if the US had heeded cytogenetic descriptions they may not have chosen chromosome 9 as a sequencing target as one arm is highly heterochromatic, full of repetitive DNA sequences, which complicated physical mapping and sequencing of that chromosome arm.
The pity is that epididymis cDNA library was missing in their sequencing target list.
SNP ascertainment bias was minimised by sequencing target DNA in at least 8 individuals from different populations [ 39].
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This was confirmed by PCR-based DNA sequencing targeting the 16S ribosomal RNA gene.
Next-generation sequencing targeting ABCA4 was applied for a widespread screening of the gene.
It seems unlikely that real-world phenotype sequencing targets will exactly match the hardest-case category.
Next generation sequencing targeted to BRAF exon 15 was utilized for mutation detection.
Whole exome sequencing targets only 1%% of the genome, and most SNPs fall outside of exomes.
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