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Forty-four of these vSAGs were selected at random for Illumina sequencing (Table 1; Supplementary Table 1 and Supplementary Figs 5 and 6).
Mutations were validated by ddPCR and cycle sequencing (Table S4) with identified VAFs similar to those found by sequencing, demonstrating high reproducibility of the data.
The median RIN score of the samples was 8.04 (range: 7.8 to 8.9) indicating the high integrity of total RNAs used for sequencing (Table 2 and Supplementary Figs. 6 9, and Supplementary File 1).
Numbered bands were further identified by sequencing (Table 3).
It was identified using molecular analysis based on the 18S rRNA sequencing (Table 1).
Results indicated that, trends of expression differences of these genes detected by qPCR agreed with those concluded by Illumina sequencing (Table 2).
The isolate was identified as Arthrobacter sp. based on morphological and biochemical characteristics and confirmed by 16S rDNA sequencing (Table 1).
Based on qPCR verification, trends of expression differences of some above genes agreed with those obtained by Illumina sequencing (Table 2).
All shRNA hairpins were confirmed by sequencing (Table S1).
Pyrosequencing confirmed all mutations detected by dideoxy sequencing (Table 1).
The N-terminal sequence of both proteins was validated by N-terminal sequencing (Table 1).
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