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Automated DNA sequencing was performed using the PRISM Big Dye cycle sequencing system and a model 373 sequencer (Applied Biosystems).
4000 PE100 sequencing system (Illumina) were performed at the McGill University and Génome Québec Innovation Center.
What was once an almost impossible task is now an automated sequencing system.
Quail, M. A., Swerdlow, H. & Turner, D. J. Improved protocols for the illumina genome analyzer sequencing system.
Library pools were purified with a 1× Agencourt RNA XP bead cleanup and sequenced on the HiSeq 2500 or NextSeq 500 Sequencing System (Illumina).
mRNA libraries were prepared according to the TruSeq RNA Illumina kit protocol and sequenced using a HighSeq 2500 sequencing system (Illumina).
Sequencing was performed at the Salk Institute Next Generation Sequencing Core on an Illumina HiSeq 2500 high-throughput sequencing system with single-end 50 bp reads.
Hovelson, D. H. et al. Development and validation of a scalable next-generation sequencing system for assessing relevant somatic variants in solid tumors.
A revolutionary third generation DNA sequencing system incorporating novel single molecule sequencing with unprecedented read lengths to reveal new biological insights in real time.
The PacBio RS II single-molecule, real-time sequencing system provides extra-long read lengths that simplify and improve genome assembly.
Using this amplicon sequencing system, we amplified a total of 234 amplicons derived from the 12 genes with multiplex PCR.
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