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Sequencing success for each primer was calculated by dividing the number of successful sequencing reactions by the total number of sequencing reactions performed for that particular primer.
Sequencing success for each partition was calculated by dividing the sum of successful sequencing reactions of the forward and reverse primer by the sum of the total number of sequencing reactions performed for each primer.
When DNA amplified, sequencing success was generally high, averaging 85% and ranging from 19% to 100%.
CBoL has reported a sequencing success of 90% for the matK region [28].
New primer cocktails were developed over the course of the study, improving sequencing success.
For H. annosum, four candidates (FG821, FG909, FG975 and FG533) yielded PCR and sequencing success.
Sequencing success was >95% for rbcL, and equally high for trnL-F when using internal primers.
The sequencing success of matK was only ∼70%, even after using two different pairs of primers.
Material collected more than 40 years ago was avoided in order to maximize the sequencing success rate.
Sequencing success averaged 91.9% overall, ranging from 72.0% for cox1 to 100% for rbcL and 23S rDNA (Table 1).
For each pathogen considered, 7 to 40% of the 10 15 best candidate genes proposed by PHYLORPH yielded sequencing success.
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