Sentence examples for sequencing specimens from inspiring English sources

Only specimens with a cycle threshold (CT) value of 30 or lower were used as we had previously noted limited success with sequencing specimens that had CT values ≥30 for RSV (CNA personal communication).

The genome could also be assembled to 190,562 bp by using Illumina data only (2.9% of the 285 million Illumina reads could be used for the MPXV assembly, compared with 45% of the 3.5 million reads from the PGM), indicating that the viral genome can be assembled by sequencing specimens of high viral load directly without previous virus propagation.

Sequences specimens from Svensson, et al. [8] were not publicly available.

According to differences in the sequences, specimens were categorized into MOTUs (molecular taxonomic operational units), using a difference of one basepair (bp) as threshold to assign a specimen to a different MOTU.

Among the 97 sequenced specimens that were subjected to AS-PCR, 77 (79.4%) were successfully amplified by AS-PCR and 20 samples (20.6%) failed to amplify after 3 repeated PCR amplifications.

Additional file 8: Gene content of our eight sequenced specimens and other published Bombycoidea mitochondrial genomes.

Ten immunity-related effector genes were differentially expressed across the sequenced specimens (Table 3).

The iMDA protocol in combination with DNA-free laboratory consumables, significantly improved the ability to sequence specimens with low levels of DNA.

Additionally, we also sequenced specimens for a subset of FLT3 ITD-positive and all other genes using the V2 Illumina chemistry with 150-bp paired-end reAdditionalional file 5).

Specimen and collection data, sequences, specimen images, and trace files are listed in the same project folder as collection data (Aquarium Imports) on BOLD.

However, a consensus is not available with regard to the specific target sequence, specimen type, extraction method, or PCR format and platform.