One of the most significant results is the sharp cost decrease for the whole genome DNA sequencing, since the psychological threshold of 1000$ for a 30X genome is about to be achieved.
Therefore, the first nucleotide at the 5' terminus of coding transcripts or of RNA genes is biased to undergo sequencing, since no hydrolysis is required to obtain a read beginning at this position.
Novel transcripts can be found by high throughput sequencing since present databases may be incomplete.
Another 2% (139) of the positions partially matched in APEX and sequencing since they yielded more than one base.
All other mismatches are due to errors in PCR or sequencing since the two strands should match up perfectly as they come from opposing sequences in the DNA.
Moreover, the use of M13-sequence tagged primers in PCR amplification assays facilitates amplicon sequencing since only one single sequencing primer (M13) is required.
Some nontarget strains were only identified to the genus level by DNA sequencing since there were no corresponding ITS or D1/D2 sequence entries in the public database.
Current studies to estimate TCR repertoire diversity are based on TCR β-chain sequencing, since TCR αβ pairing can be assessed only at the single-cell level.
