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This sequencer does not rely on fluorescence, chemiluminescence, or enzyme cascades for sequencing signal detection.
In spite of the high sensitivity and productivity of NGS sequencers, their signal detection system is not sensitive enough to measure the sequencing signal originating from a single molecule.
Input sequencing signal was plotted over same regions.
This substitution was found to further improve DNA sequencing signal and efficiency.
Those clones which suffered a systematic drop on sequencing signal after poly-A tails were sequenced from 5' end.
Those clones that suffered a systematic drop on sequencing signal after poly-A tails were sequenced from the 5′ end.
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Each dataset includes the genome-wide sequencing signals and regions of statistically enriched signal (peaks).
As expected, T827N3 and T827N4 resulted in greater DNA sequencing signals than the completely degenerate version T827N5 (data not shown).
Next-generation sequencing signals, however, are suitable for studying heterogeneity and characterizing subclonal components by their collection of specific markers.
To formally describe and analyze the control sequences, Signal Interpreted Petri Nets are utilized.
Excitation sequence signal is active low, and TTL level for direct interfacing to the microcomputer and gate array.
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