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The plasmid inserts were verified by sequencing (Sequencing Core Facility CeBiTec, Bielefeld, Germany), and the plasmids were used to transform C. glutamicum ATCC 13032.
All cDNAs were checked by automatic sequencing (Sequencing Facility, Rennes Hospital, France).
Since the advent of large-scale DNA sequencing, sequencing error has periodically emerged as a research topic of primary interest in genomics [4], [7], [8].
Although the complete 16S mtDNA sequences for most of the species analysed is available in the public domain, we regenerated the target sequences for the thirteen mammal species used using conventional dye-labelled sequencing (Sequencing reactions and analyses performed on Applied Biosystems platforms by Macrogen, Korea).
454 sequencing: Sequencing was performed according to manufacturer's protocol.
BRL performed sequencing, sequencing analysis and MAR assays and wrote the manuscript.
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YJ and CG assisted with sequencing, sequence analysis and annotation.
SJP carried out DNA sequencing, sequence assembly, sequence analysis, other molecular genetic analyses and co-wrote the manuscript.
GM, AC, BSand SSwere implicated in BAC clone sequencing, sequence assembly and verification of assembled sequences.
Targeted re-sequencing: sequencing of a defined (targeted) region of the genome; helpful in identifying disease variant(s) when a linkage region has been established.
One hundred and six soybean lines with carbohydrate phenotypes (sucrose, stachyose, and raffinose) and whole genome re-sequencing (sequencing depth approximately 15X) data were obtained for soybean SWEET genes from Soybean Genetics and Genomics Laboratory at the University of Missouri (Valliyodan et. al. Unpublished).
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