Exact(7)
We selected 140 unrelated IS cases of European ancestry with severe deformity (spinal curves measuring ≥40°) for the exome sequencing screen.
Two of these (mutations in the catalytic domain of AKT2 (D399N) and the regulatory domain of AKT3 (E438D)) were previously reported in a sequencing screen of 123 genes in 41 primary endometrial cancers (Dutt et al, 2008).
Seven sRNA candidates were obtained by sequencing screen of 2,500 clones from the library and four of them were confirmed to be sRNAs by Northern hybridization, which were named sRNA- Xcc1, sRNA- Xcc2, sRNA- Xcc3, and sRNA- Xcc4.
ARHGEF10 has emerged as a candidate colorectal cancer gene in the sequencing screen carried out by Wood et al. [ 56], and, in conjunction with our discovery of a heterozygous deletion of ARHGEF10 in DU4475 with mutation of the remaining copy, this makes it a strong candidate for the 8p23.3 tumour suppressor.
We have now carried out a PCR and sequencing screen to look for the presence of the T to G mutation in a population of migratory marine fish from Rabbit Slough AK. (This work was carried out in part by Shannon Brady, who has now been added a co-author of the manuscript).
A cDNA library was constructed with the low molecular weight RNA prepared from the Xcc cells grown to exponential phase in the minimal medium XVM2 and seven sRNA candidates were obtained by sequencing screen of approximately 2,500 clones randomly selected from the library.
Similar(53)
We obtained from dbEST a number of mismatches roughly 5 times higher than the genome wide sequencing screens.
Moreover, RNA sequencing screens identified EAAT1 and EAAT2 as targets of TDP-43 (16, 16).
In addition, Johnson et al. carried out a sequencing screening of exon 5 of the CD20 gene encompassing the epitope of rituximab.
Astro Chase, with its smooth scrolling and attractive launch sequence screen, was one of the first third-party games to show what the 8-bit Atari was capable of.
Screening for these changes is warranted in individuals who have a negative germline sequence screen of MLH1 and loss of MLH1 expression in their tumor.
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