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In order to characterize the effect of sequencing scale on the statistical results obtained, we generated resampled data sets with various numbers of miRNAs.
The small RNA sequencing resources are listed in Additional File 8. Sequencing abundances were normalized to the average sequencing scale for all samples in a given database unless the values had already been normalized using the original data generators.
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The explanation is obvious - the cost of sequencing scales more or less linearly with the number of basepairs to be sequenced.
To calculate such values which we refer to as "sequencing scaled microarray intensities" or "SSMIs"—we tried several approaches (see Additional file 3).
As genomic sequencing scales up from a few thousand tests annually to millions of tests a year ordered by physicians from all sorts of backgrounds...does anyone imagine that a physician could adequately counsel 200 patients in advance about 2 dozen rare disorders in order to preserve the right of refusal for the 1 who might receive a frightening result?
The cost of phenotype sequencing scales according to the size of the genome (or region of interest) being sequenced.
We took NCBI GEO (GSE11014 and GSE13152) as main reference for small RNA expression because their sequencing scales are the largest.
These sequencing scaled microarray intensities (SSMIs) provide more reliable, quantitative estimates of absolute expression levels for many genes when compared with unscaled intensities.
Architectural style is similar at individual sequence scale for comparable positions within each composite sequence set and each composite sequence.
These observations are helpful in predicting the location of porous and potential sealing bodies and baffles to fluid flow at the intra-high-frequency sequence scale, and ultimately they are useful for both petroleum exploration and production.
3) The closer the points to the diagonal, the smaller the sub-sequence scale it represents.
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