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Next-generation sequencing refers to new sequencing techniques which overcome most of the limitations of the methods described above.
Next-generation sequencing refers to a whole suite of different techniques for rapidly sequencing huge numbers of base pairs.
BAC-end sequencing refers to the bidirectional end sequencing of the genomic DNA insert with the help of universal priming sites in the cloning vector.
Amplicon sequencing refers to ultradeep sequencing of PCR products obtained from one or several specific targets in order to determine, for example, genetic variations contained in precise portions of the genome.
"SOLiD ORA" refers to the transcripts determined from our experiment using ORA, "Tiling arrays" refers to the data reported by Xu and colleagues [ 10], "5'-RACE" and "Illumina sequencing" refers to data reported by Nagalakshmi and colleagues [ 9] and "SOLiD Cufflinks" refers to the transcripts reported in our experiments and analyzed using Cufflinks [ 20].
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For an overview of the number of amplicons that required Sanger sequencing, refer to Additional file 2: Table S2.
The climactic sequence refers to the scene in "Citizen Kane" in which Kane, left by his wife and desperately alone, tears apart a room in Xanadu, the mansion he built as a monument to himself.
The sequence refers to the order in which the elements of a program are delivered across time.
The number of sequence refers to the respective number of unique sequences in the specific segment.
Ref. sequences refers to the number of sequences in the alignment.
(1) The start base position of the interspersed repeats and palindromic sequences refers to the cv.
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