The course was taught using a 454 FLX titanium sequencer, and covered the entire DNA sequencing process.
The sequencing process took several days.
The normal distribution of GC content was indicating non-contaminated in sequencing process (Fig. 2c).
For a more detailed explanation and schematic of Complete Genomics's sequencing process, click here.
One aliquot was used for the library preparation and sequencing process, the second one was stored as a backup.
We perform extensive quality assurance on our equipment and quality control of samples throughout the sequencing process.
To guard against the incorporation of erroneous data derived from DNA damage or contamination, the PCR amplification and sequencing process was replicated twice for each sample.
For those samples with negative results after the sequencing process, we used multiplex ligation-dependent probe amplification (MLPA) method for CNV analysis.
Then DNA nanoballs (DNBs) were generated with the ssDNA circle by rolling circle replication to enlarge the fluorescent signals at the sequencing process as previously described36.
It is typically challenging for scientists to distinguish small mutations from random errors generated during the sequencing process, especially in repetitive portions of a genome.
Throughout the DNA sequencing process, the PacBio RS uses advanced collection optics to record light pulses emitted as a byproduct of nucleotide incorporation.
