Sentence examples for sequencing procedure to from inspiring English sources

Finally, the potential role of the DNMTs in determining D-loop methylation status was investigated by applying the bisulphite sequencing procedure to DNA samples from wild-type (wt) and Dnmt1 −/−, Dnmt3a −/−, and Dnmt3b −/− mouse embryonic stem (ES) cells.

Here, by applying a strand-specific RNA sequencing procedure to different stages of postnatal development, we have obtained one of the most complete and dynamic pictures of the human prefrontal cortex transcriptome.

The Number-Letter Switching condition involves a sequencing procedure similar to that of the classic TMT.

An assembly consisting of more than one contig indicates that the sequencing procedure failed to generate sufficient sequence reads from the DNA residing between contigs (i.e., gaps).

Moreover, since the sequencing of the targeted loci is still based on PCR amplification, some of the errors will be similar to those found in the traditional cloning/Sanger sequencing and can originate during the first target-specific PCR or the 454 sequencing procedures due to polymerase nucleotide misincorporation, chimera formation, or both.

In this study, a sequencing procedure was used to sequence simultaneously and rapidly multiple archived samples using a single standard protocol.

For economic reasons, these sequences are obtained by bulk sequencing; that is, the sequencing procedure is applied to a diverse sample of the HIV population.

Since the shotgun sequencing procedure is expected to provide a random sampling of the different cDNA strands, most of the reads will hit the most abundant genes.

Regions with less significant PfamA matches (10−5100 residues) with no significant PfamA hits were analyzed with the consensus sequence procedure [20] to decide whether they are significantly related to known domain families.

DNA prior to sequencing procedure was diluted in nuclease-free water.

It could be that the minor alleles are artefacts of the cloning and sequencing procedure; chimeras are expected to occur in amplifications of closely related sequences [ 43].