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The sequencing preference may lie in two aspects.
We chose 40 nucleotides, 20 bp upstream, and 19 bp downstream to estimate the sequencing preference.
Then we developed the PDEGEM, a Positional Dependent Energy Guided Expression Model to take into account the sequencing preference.
For a given position of a transcript, we use the nucleotide sequences nearby to evaluate the sequencing preference of this position.
Furthermore, we also attempt to use 20, 60, 80, and 120 nucleotides to characterize the sequencing preference (See Additional file 1 for more details).
As for positional weight, the results show that the nucleotides in the middle portion of the sequence have larger effects on the sequencing preference.
Similar(42)
Beyond sequence preference, chromatin features dictate the repertoire of these genome-modifying enzymes.
The structural basis of the amyloid formation and the sequence preference are unknown.
There is limited evidence to suggest a slight GC sequence preference.
The 2935 motifs were assigned k-mer scores reflecting the degree of sequence preference with respect to 3-mer (n = 64) and 4-mer (n = 256) words.
Apparently the star activity of DraIII has a sequence preference for the central three nucleotides, whereas the canonical cleavage activity does not (Grosskopf et al., 1985).
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