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AGS oversaw the 3′ cDNA library construction and sequencing portions of the work.
This limitation was partially addressed by sequencing portions of the MAP sheep isolate 397 genome.
Diagnosis was established by using polymerase chain reaction of fungal DNA followed by sequencing portions of translation elongation factor 1 alpha (TEF1 α) and beta-tubulin (BT2) genes.
Sequencing of the entire mitochondrial genome provides higher resolution and discrimination power than is currently possible with only sequencing portions of the non-coding region of the mitochondrial genome (for high information content) or by targeted analyses (for a few SNP or deletions noted in the coding region).
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Large rearrangement analysis components of the 25-gene panel were validated on additional positive samples, along with the 100 samples used for the sequencing portion of the validation which did not contain large rearrangement mutations.
Scientists from New Zealand and Italy sequenced portions of mitochondrial DNA from 96 penguin bones that were up to 6,400 years old, and from 380 blood samples of living penguins.
Incidence of APRs within TcIERs is not special to biotherapeutics, self-TcIEs from human proteins, involved in various diseases, also contain predicted APRs and experimentally proven amyloid-fibril-forming peptide sequence portions.
In addition, the C-terminal sequence portions of the collected T3SS effectors were included in the negative training set.
Expressed sequence tags (ESTs) are sequenced portions of messenger RNA.
The mitochondrial and the ITS data sets contain sequence portions that are highly variable.
Vector and PCR primer sequence portions were removed from cDNA sequences using Geneious software (Biomatters Ltd., Auckland, New Zealand).
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