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The project was divided into mapping and sequencing phases, with the idea that maps of markers ordered along human chromosomes would guide the sequencing strategy.
An overall novelty of greater than 45% remained throughout the sequencing phases of the non-normalized, normalized and subtracted libraries (Fig. 3).
For example, the TGP conducted both painstaking computer simulations and pilot sequencing phases involving hundreds of genomes to aid in designing the full-scale project [ 6, 7].
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One of the things I do is serve as the project manager overseeing the sequencing phase of the genome project.
In the initial random sequencing phase, approximately 6-fold sequence coverage was achieved from the two libraries (each library was sequenced to 3-fold coverage, respectively).
Moreover, the positive effect of better assignment and classification of long reads comes with higher costs and workload in the sequencing phase.
The shotgun sequencing phase for this genome required ~35,200 sequence reads to reach 8-fold coverage [ 18].
First, the sequencing phase of genome projects typically results in hundreds to thousands of contigs rather than chromosome-length assemblies.
In the sequencing phase of the Arabidopsis Genome project, it was agreed that each group would continue sequencing up to the region containing intractable centromeric repeats.
The sequencing phase may consist of 20 50 cycles (Illumina, 2008b), limited by the probability (or the corresponding quality score) with which each nucleotide can be identified.
Microsatellite marker development was conducted simultaneously with the sequencing phase of the project through hybridization of (GT 11 and (GA 11 probes to high-density filters representing 27,648 clones from the library.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com