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The technical reproducibility was assessed by two independent meDIP experiments and sequencing performances for RJF muscle.
To compare the mtDNA sequencing performances of the 454 and Sanger platforms, we used defined metrics [11].
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The results were then compared to the sequencing performance observed when no oligonucleotide spiking was performed.
The foregoing suggests similar sequencing performance for both systems.
Sequencing performance demonstrated that the Precision ID Ancestry Panel is effective and robust.
The kit has been optimized to provide outstanding sequencing performance and uniform coverage of the entire transcript.
Topographic particularities in areas such as the hippocampus, pre-motor cortex, and motor cortex in DS individuals with moderate deficiency levels may be considered determinants for differences in sequencing performance.
As an initial test of RAD tag sequencing performance for genetic mapping, we created RAD tag libraries from parental RS and BP genomic DNA digested with SbfI, which recognizes an 8-nucleotide sequence (CCTGCAGG).
Sequencing performance according to sample type is displayed in Table 3.
The sequencing performance and library quality was further assessed using RNA-SeQC v1.1.7 [ 15].
Custom scripts were used to assess sequencing performance, library quality and alignment quality.
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