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The DNA is then purified and subjected to library construction reactions, where the fragment ends are made double stranded, phosphorylated and A-tailed before sequencer-specific adaptors are ligated to allow amplification and sequencing of the sample.
The adaptors provided priming sequences for amplification and sequencing of the sample library fragments as well as 'the sequencing keys', a short sequence of 4 nucleotides.
Note that WRVs are independent of GC content and depth of sequencing of the sample.
Preparation and sequencing of the sample was performed according to the manufacturer's instructions.
The coverage plots were then generated using Samtools (Li et al. 2009) and standardized by dividing the coverage at each base by the number of million reads generated in the sequencing of the sample.
The fragment ends were made blunt and short adaptors which provide the priming sequences for both amplification and sequencing of the sample library fragments were ligated onto both ends.
Similar(52)
By April, scientists at Illumina and TGen, a nonprofit research institute, had completed the genetic sequencing of the samples.
JC-R carried out cloning and sequencing of the samples.
However, the sequencing of the samples could not confirm a mutation despite several sequencing attempts.
Library preparation and RNA sequencing of the samples were performed as described previously.
In addition, exhaustive deep sequencing of the samples would be required to fully reveal the extent of sequence overlap.
More suggestions(15)
timing of the sample
continuum of the sample
ageing of the sample
sequences of the sample
ordering of the sample
steps of the sample
linking of the sample
sequencing of the top
sequencing of the acrR
sequencing of the coding
sequencing of the virus
sequencing of the peptide
sequencing of the father
sequencing of the spot
sequencing of the representative
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