Sequencing of the coding and anti-coding strand of the oprD PCR products necessitated two additional internal primers (Table 6).
Sequencing of the coding and near-coding regions of genes relevant to each patient's clinical presentation revealed 37 sequence variations of known or uncertain pathogenicity in 25 patients.
PCR amplification, and Sanger sequencing of the coding and near-coding regions of selected genes, were performed according to standard protocols.
DNA sequencing of the coding and near-coding regions of genes relevant to each patient's clinical presentation revealed 37 sequence variations of known or uncertain pathogenicity in 9 genes from 25 patients.
A high-throughput gene study involving solution capture and sequencing of the coding exons of 20,000 protein-coding genes identified new cancer loci, including genes encoding enzymes that demethylate/methylate key lysine residues in histone H3, which is implicated in transcriptional control by regulating chromatin structure [ 23, 25].
This study involved cloning and sequencing of the coding regions of all 10 Toll-like receptor (TLR) genes of goat.
Comprehensive sequencing of the coding regions of all genes (Whole Exome Sequencing or WES) is more affordable, but still has high technical requirements that are an obstacle to its use as a diagnostic method in routine clinical practice.
Full-germline DNA sequencing of the coding regions was performed on three HNPCC genes, MLH1, MSH2 and MSH6.
The analysis of rare variants of the genes was performed by sequencing of the coding portion of the gene and 5'-untranslated region (5'UTR) with the Sanger method.
Sequencing of the coding sequence of PAX2 showed no evidence for disease causing mutations in sheep.
Although haplotype tagging polymorphisms are deliberately selected to have low levels of pairwise linkage disequilibrium, we also included additional SNPs identified in the sequencing of the coding region.
