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The ability to easily develop custom panels of SNP markers for interrogating genes or genome regions of interest will complement modern genetic strategies that identify candidate variants through deep sequencing of population samples.
In this study, we performed high-quality Sanger sequencing of population samples from both D. melanogaster and D. simulans and found that all eight genes involved in GSC regulation studied here reject a neutral model of evolution in at least one test and species (Tables 3 and 4).
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Further studies, such as single-cell RNA sequencing of populations of interest, are warranted to further characterize the heterogeneity of these populations.
Even whole-genome sequencing of populations, the ultimate genome diversity survey tool, reveals at most the potential of a population to express various phenotypic features.
A recent study has quantified the temporal dynamics of point mutations over 1000 generations by deep sequencing of populations (Lang et al. 2013).
With increases in the throughput of sequencing technologies, the approaches applied for genetic variation analysis start shifting from the usage of SNP-based genotyping assays to the direct sequencing of populations (Hamilton and Buell 2012).
Sequencing of populations at different evolutionary times shows that the number and type of mutations are compatible with other laboratory evolution experiments, which are characterized by competition between variant clones (Barrick et al. 2009; Bachmann et al. 2012).
The main sequence of Population II stars extends downward to fainter, redder stars in much the same way as in the spiral-arm Population I stars.
Schoen (2014), building on the birth-death model in Schoen (2013), presented a multistate projection approach based on the idea of "intrinsic linkages," i.e., linear relationships between the dominant right eigenvectors of projection matrices and the sequence of population compositions.
Despite such mutation, the number of total mutations fixed in the consensus sequence of population RAp35 was very high (1.13 mutations/passage, Figure 6) when compared with its ancestor (0.2 mutation/passage).
There is an increasing need to conduct spliced and unspliced alignments of short transcript reads onto a reference genome and estimate minor allele frequency from sequences of population samples.
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