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The MetaSim software [41] was used to create two such datasets to test IHAT over fragments 1000 bp (typical Sanger sequencing length) and 350 bp (typical 454 GSFLX pyrosequencing length) in length respectively.
Millions of raw reads were generated with a sequencing length of 49 bp.
The total sequencing length was 75,920 Mb, covering ∼195-fold of the whole genome.
Each tunnel generated millions of raw reads with a sequencing length of 49 bp.
Each tunnel will generate millions of raw reads with sequencing length of 35 bp.
The lower cutoff selected should therefore be well-above the desired sequencing length.
Similar(35)
Sequence length is over 20. .
N indicates the sequence length.
The maximum sequence length was 54.
Isothermal UV absorbance spectra has a limitation of sequence length.
Sequence length was automatically adjusted to maintain 87% correct performance.
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