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This limitation might be overcome by deeper sequencing (higher coverage of the position in question).
We demonstrate the advantages of this approach on a lung tumor [patient 330 in Zhang et al. (2014)] that had multiple samples sequenced using both whole-exome and targeted deep sequencing (higher coverage) data.
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These sequencing methods have been called next generation sequencing, high-throughput sequencing methods, or second-generation sequencing.
In de novo whole-genome sequencing, high coverage may compensate for erroneous sequences.
This large number of short gaps is consistent with the limitations of sequencing high-AT content genomes.
To apply NGS to whole transcriptome sequencing, high-quality RNA must be reliably obtained, often from diverse taxa.
NGS technology was successfully applied to de novo and re-sequencing of entire prokaryotic genomes [ 9] and to re-sequencing higher eukaryotes including humans [ 10- 13].
In Blue, sequences higher than 3000 bp.
The plantsUPS is the first platform concerning UPS in seven sequenced higher plants.
The neem genome bears fewer repetitive elements compared to other sequenced higher plants.
With regard to HSP sequences, higher bootstrap values were obtained with protein sequences than with nucleotide sequences (data not shown).
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