Sentence examples for sequencing failure from inspiring English sources

Sequencing failure may be due to suboptimal primers, but up to 5 different primers were assayed to confirm GDNF without any success.

In other words we do not track metrics as to whether eight of eight clones were correct, or if a non confirming clone is do to a cloning failure, a sequencing failure or a related issue.

A high sequencing failure rate was also found in the human homologue of the mouse SMAD4 coding exon 7.

Rates of contamination by exogenous DNA and sequencing failure were minor compared with the precision in quantifying and identifying prey composition.

This kind of sequencing failure was demonstrated when the DNA of a putative novel subtype of HPV-87 was amplified by the first nested PCR in the presence of competing non-HPV DNAs.

This high degradation of FFPE used material can also explain the higher Sanger sequencing failure rate described in other studies using a larger PCR product for analysis [ 24, 27].

Excluding sequencing failures our success rate, defined as identifying a base perfect clone, is >90%.

By contrast, the use of primer Pro1F yielded sequencing failures due to the co-sequencing of different heteroplasmic variants linked to a 82 bp tandem repeat region located at the 5' region [18].

Sequencing failures: ST.

Sequencing failures were attributed to low BAC DNA yield.

Repeated sequencing failures necessitated cloning of the amplified fragments from 'Kaiser Wilhelm', however.