In any strategy, it is important to have a high quality reference assembly available for annotation and re-sequencing endeavours.
Cancer-exome-sequencing endeavours have recently revealed that core components of the KDM2B/PRC1 complex, including KDM2B itself and BCOR/L1, are frequently mutated in a range of cancers, particularly leukemias (Brookes et al., 2012; Grossmann et al., 2011; Pasqualucci et al., 2011; Zhang et al., 2012).
It is written as a lifelong series of journals kept by Mountstuart, a writer whose life (1906 1991) spanned the defining episodes of the 20th century, crossed several continents and included a convoluted sequence of relationships and literary endeavours.
Thus, the sequencing efforts are community-driven, community-funded endeavours, the results of which are proving to be of immense benefit for the communities that developed them as well as many other researchers in the plant sciences field.
With the on-going effort in porcine genome mapping and sequencing [ 20], the capacity to achieve this endeavour is now available.
Sequencing of mitochondrial genomes has become an important endeavour for providing molecular resources for population genetic and phylogeographic studies [ 1- 3].
Due to the expected size of the P. patula genome (ca. 22 Gb) [ 41], sequencing and assembly of the genome would be a costly and challenging endeavour.
Although large-scale studies have mostly used random approaches to introduce variability in the synthetic sequences to be interrogated (Dvir et al., 2013; Quan et al., 2011), similar endeavours have greatly benefited from following well-established design of experiments (DoE) (Allert et al., 2010; Antony, 2003; Kosuri et al., 2013; Sharon et al., 2012; Smith et al., 2013).
These endeavours have drastically increased the amount of miRNA and mRNA data at both expression and sequence levels.
