The peptide should have the following features: unique amino acid sequence, efficient protease digestion, appropriate LC retention time, and good MS sensitivity.
For established and well-studied genomes, finishing builds by additional sequencing yields diminishing returns, and novel, targeted and highly sequence-efficient methodologies such as Strand-seq and BAIT can play a crucial role in completing these genomes.
However, the advent of next generation sequencing provides efficient and cost effective tools for variant analysis even in this highly complex genome.
As ever more protease sequences are uncovered through genome sequencing projects, efficient parallel methods to discover the potential substrates of these proteases becomes crucial.
Next-generation sequencing enables efficient development of large numbers of SSR markers at lower costs.
Transcriptome sequencing enables efficient high-throughput discovery of single nucleotide polymorphism (SNP) markers for a complex polyploid species.
With the growth of large community annotation projects due to the rapid progress of the next-generation sequencing technologies, efficient and optimized genomic data management is critical.
Genetic testing using targeted capture followed by next-generation sequencing was efficient, cost-effective, and enabled a molecular diagnosis in many refractory cases.
The CRISPR/Cas9 protein requires a Cas9-specific protospacer-adjacent motif (PAM) sequence being present at the 3′-end of the targeted sequence for efficient binding and cleavage.
The CU also internally communicates with every module in sequence for efficient data transfer with the card.
