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Using Sanger sequencing, editing levels of known conserved targets of A-to-I RNA editing were analyzed from the cerebral cortex of rats used in the RNA-seq: Gabra3, Cyfip2, Kcna1, Flna, and Blcap (which has three editing sites, known as Y/C, Q/R, and K/R), and the newly identified site in Cog3, using primers given in Table 1.
EEB and XZ performed the DNA sequencing, editing, and alignment.
The methods for generating a cpDNA consensus sequence, sequencing editing, and alignment follow those employed for the nrDNA cistron (Fig. 2).
Similar(57)
Therefore, there may be some sequence editing errors in the rabbit CXCR1 and/or CXCR2 gene.
Sequences were aligned using CLUSTAL W [ 52] and were manually checked in the BIOEDIT sequence editing program [ 53].
"A mook, what's a mook?" They fight, in a sequence edited to the rhythms of Please Mr Postman.
We subsequently manually edited the alignments by realigning the first 45 bp of short sequences ("edited" matrices).
Eighty-seven BAC-ends of PWD/Ph clones were sequenced, edited, and aligned with mouse C57BL/6J (B6) genome.
ST extracted minicircle sequences, predicted edited sequences of maxicircle genes, performed all analyses of minicircle sequences and drafted the manuscript.
Sequence editing and inspection were done with Sequencher V5.4.5.
In this work, sequence editing strategy was employed to improve the performance of AtGUS.
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