Though she ascribed these problems to "sequencing difficulties," they seemed to arise from her failure to empathize with the characters, to follow the intricate play of motive and intention.
While none of the proposed markers provided unique identifiers for all species tested, nrITS showed the most promise in terms of variability, although sequencing difficulties remain a drawback.
Despite these issues, nrITS had sufficient variation to correctly identify individuals to species for the 27 individuals initially tested plus 4 additional species represented by sequences downloaded from GenBank (due to sequencing difficulties for Zamia and lack of fresh tissue samples for Bowenia).
Another reported strategy to alleviate sequencing difficulties is to include mismatched bases within the shRNA stem [ 3, 11].
However, the ends of some sequences, for both COI and CYB, were shorter because of sequencing difficulties and contained a series of Ns.
While we did see a reduction in sequencing difficulties when mismatches were present, we also observed a correlation between increasing mismatches and decreasing gene suppression activity (Fig. 5).
It often happens that the collection of input MUL-trees are labeled by slightly different multisets (due e.g. to sequencing difficulties or lack of sampling).
The ESTs showing best match to a specific NBS-LRR-encoding or related gene but with sequence identity less than 97% due to obvious sequencing difficulties were also counted.
For 9 out of the 12 CBF/ DREB1 genes, one or two internal primers were needed to ensure full sequence coverage and/or overcome sequencing difficulties caused by simple sequence repeats [(A)n or (T n] present in 5′- or 3′-non-coding flanking regions (Additional file 10).
Motor or automatic sequencing difficulties, such as difficulty in learning to tie shoes.
An integrated indicator named sequence difficulty degree (SDD) is proposed by combining these five metrics to intuitively represent the sequence-level tracking difficulty of the infrared image sequence.
