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Though she ascribed these problems to "sequencing difficulties," they seemed to arise from her failure to empathize with the characters, to follow the intricate play of motive and intention.
While none of the proposed markers provided unique identifiers for all species tested, nrITS showed the most promise in terms of variability, although sequencing difficulties remain a drawback.
Despite these issues, nrITS had sufficient variation to correctly identify individuals to species for the 27 individuals initially tested plus 4 additional species represented by sequences downloaded from GenBank (due to sequencing difficulties for Zamia and lack of fresh tissue samples for Bowenia).
Another reported strategy to alleviate sequencing difficulties is to include mismatched bases within the shRNA stem [ 3, 11].
While we did see a reduction in sequencing difficulties when mismatches were present, we also observed a correlation between increasing mismatches and decreasing gene suppression activity (Fig. 5).
However, the ends of some sequences, for both COI and CYB, were shorter because of sequencing difficulties and contained a series of Ns.
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An integrated indicator named sequence difficulty degree (SDD) is proposed by combining these five metrics to intuitively represent the sequence-level tracking difficulty of the infrared image sequence.
However, using conventional MRI sequences, difficulty in differentiating benign from malignant lesions may arise when malignant and benign lesions share certain morphologic and contrast-enhancement characteristics.
For PAML analyses, taxa that have diverged more than ~100 MYA may lead to decreased analytical power due to highly divergent sequences, difficulty in sequence alignment, and saturation of substitutions [ 56- 58]; therefore, only taxa that have diverged less than 100 MYA were included in the data set.
The diversity of potential regulatory sequences, difficulty predicting biologic regulation based solely on a consensus miRNA binding site, and the increasingly apparent need for confirmation in living cells indicate that additional, cell-based studies should be used in the future to address transcriptome regulation by miRNA.
Meanwhile, the high degree of similarity and duplication of TE sequences presents difficulties in genome sequencing, annotation and analysis.
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