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The Ion Torrent Personal Genome Machine (PGM) (Life Technologies), launched in early 2011, has the highest throughput compared with 454 GS Junior (Roche) and Miseq (Illumina), thus making the high-throughput sequencing cost effective and time saving [ 18].
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Transcriptome sequencing is cost effective compared to traditional Sanger sequencing method, and is a suitable alternative to whole genome sequencing as it can provide information of transcribed portion of genes at a lower cost [ 22].
In summary, clinical exome and genome sequencing are cost effective methods for identifying the molecular bases of genetic conditions.
The option of sequencing the entire chloroplast genome (Nock et al. 2011) for better resolution can also be considered, now that next-generation sequencing methods have made whole-genome sequencing much more cost effective.
Recent advances in sequencing technologies have produced cost effective methods to generate high sequence coverage of large genomes [ 3, 4].
Currently, genomes are frequently sequenced by cost-effective next-generation sequencing (NGS) technologies.
However, avoiding sequencing duplicate reads to begin with saves resourses and makes the sequencing procedure more cost effective.
Exome sequencing offers a cost effective method for comprehensively screening variations in complex samples.
The utilisation of barcode tags to de-convolute pooled PCR products in multiplexed, variation screening via Illumina sequencing provides a cost effective method for SNP genotyping and mutation detection and, potentially, markers for causative changes, even in polyploid species.
We have developed a simulation approach to help determine the optimal mixture of sequencing methods for most complete and cost effective transcriptome sequencing.
Customized Perl scripts were developed to analyze the sequence information, with the recently released Velvet software including the Columbus module for sequence assembly, to select an initial set of ~6,000 candidate SNPs from those sequences in a cost effective manner.
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CEO of Professional Science Editing for Scientists @ prosciediting.com