Surprisingly, [ 13] found that shorter reads are better for IE when the total number of sequenced bases (as a rough approximation for sequencing cost) is fixed.
With the richer information from WGS and fast dropping sequencing cost, WGS may soon replace WES as the main deep-sequencing technology for genetic studies.
The sequencing cost $50,000.
The first whole human genome sequencing cost a whopping $2.7 billion.
Its complicated library construction procedure and high sequencing cost are two main limiting factors for the use in individual laboratories.
However, with reduction in DNA sequencing cost, high resolution QTL mapping is now possible using SNP markers.
With the continuous decrease of sequencing cost, RNA-seq become the most prevalent method for identification of circRNAs.
A low sequencing cost of around US$1000 per genome has now rendered large population-scale projects feasible.
The success rate in isolating flanking regions is also maximized in the latter events (unpublished), thereby reducing the overall sequencing cost.
With the rapid falling of sequencing cost, ∂a∂i program has been used in several model and non-model species for the whole genome resequencing data.
As the sequencing cost for SBS is further reduced in the future, SBS will likely become a routine transcriptomic analysis for many biological experiments.
