Sentence examples for sequencing context from inspiring English sources

PCR amplification during next generation sequencing library preparation is not equally efficient across all genomic regions and depends on sequencing context.

Finally, flow cell lane, machine cycle (base position within the read), sequencing context (preceding and current nucleotide) or other technical aspects may influence base quality scores, which help characterize the quality of the bases in the individual reads.

Typically, the Ti/Tv ratio is lower in the newly discovered SNPs than that in known SNPs because of a combination of residual false positives, a relative deficit of transitions due to sequencing context bias, and an apparently higher transition ratio at lower frequency variation [ 23].

The observed value in the case of novel SNPs at 1.96 is lower than the expected range of ~2.0-2.1 ~2.0-2.1n be due thisne or a canbenation of the following reasons: possibility of residuel false positoves, poneibility or a relative deficombinationsitiofs due thesequencing context bias, or because ofollowingareasonshigher transition ratio often associated with low frequency variants [ 20].

In order to increase the resolution of the derived mutational signatures, substitutions were examined using their immediate sequencing context.

This mutational pattern and its immediate sequencing context are consistent with the activity of the error-prone polymerase η during somatic hypermutation [ 36,80].

RNA sequence context matters in the termination of protein translation.

Each domain displays a distinct preference for the sequence context of the phosphorylated residue.

This efficient recognition was surprising, because the uAUG codon of AtMHX has a weak sequence context.

However, that work was not fully exploited in an image sequence context as yet.

In both experiments, complete loss of activity demonstrates the importance of sequence context in determining whether substitutions are functionally acceptable.