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A typical amplicon design for targeted sequencing consists of small overlapping amplicons that tile the target region as shown Figure 1.
Oxidative bisulfite sequencing consists of the selective oxidization of 5-hmC to 5-formylcytosine prior to bisulfite conversion (Booth et al. 2012).
Amplicon sequencing consists of the polymerase chain reaction (PCR) amplification of a limited number of the genomic regions of interest (amplicons) followed by high-throughput sequencing (Supplementary Fig. S1A) (Beadling et al., 2013).
Although relatively expensive exome sequencing consists of in-depth sequencing of nearly all the coding exons, the amplicon sequencing technique aims at sequencing a limited number of genes (from several dozen to several thousand exons) at an extremely low cost.
The currently known protein space, which is a part of the protein universe that has been revealed by DNA sequencing, consists of more than 16 million protein sequences in a non-redundant (nr) database (December 8 , 2011 and its size is rapidly increasing due to recent technological advances [ 4, 5].
As shown in Figure 1b, the output from human whole genome sequencing consists of both nuclear DNA (nuDNA) and mitochondrial DNA (mtDNA) molecules, thus it is convenient to assess mtDNA copy number from WGS dataset and can be an alternative to real-time PCR assays.
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Pooled sequencing data from both Illumina and 454 sequencing consisted of approximately 775 million reads.
Our sample for DNA sequencing consisted of a total of 38 Dorylus s.l.l
Sequencing consisted of one lane, with a paired end run of 51 cycles.
The oldest of these is EST sequencing, consisting of end reads from cDNA clones.
The International Trade sequence consists of two courses: 720 and 721.
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