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For each sample, at least 20 µg of total RNAs was sent to the Beijing Genomics Institute for Illumina sequencing (commercial service).
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Plasmid was purified using Gene Jet Plasmid Miniprep Kit (Fermentas, St. Leon-Rot, Germany) and clones sequenced (commercial sequencing facility of GATC Biotech, Konstanz, Germany).
The sequenced commercial cultivars were selected to represent a subset of the lupin breeding history released from 1973 to 2007.
Samples were sequenced using commercial sequencing chemistry.
The inserts in 247 plasmids from the ADAM libraries were sequenced by commercial sequencing facilities.
Purified PCR products were then sequenced using commercial sequencing services (First Base Sdn Bhd., Malaysia).
Positive colonies were screened out and sequenced by commercial company (Invitrogen).
The same samples as above were sequenced using commercial kits and protocols provided by Life Technologies, Carlsbad, USA.
Because the BAC clone sequences did not completely assemble into a single contig, subclones were generated from DNA of each BAC clone as described in Terefe-Ayana et al. [ 35] and Sanger sequenced using commercial sequencing services.
The sequences of the DNA constructs were verified by sequencing using a commercial service from Macrogen (Rockville, MD).
We prepared the fragmented DNA for sequencing using the commercial Illumina protocol for TruSeq DNA library preparations (Illumina, San Diego, CA).
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