Sentence examples for sequencing assays from inspiring English sources

However, the large amounts of raw data produced by next-generation sequencing assays and the need for complex bioinformatics analyses present unique challenges.

We report an approach to provide effective multianalyte controls for next-generation sequencing assays, referred to as the control plasmid spiked-in genome (CPSG).

We compared TI-NGS with commercial genotype- or subtype-specific Sanger sequencing assays, and found that TI-NGS both confirmed most of variants called by Sanger sequencing and avoided biases likely caused by PCR primers used in Sanger sequencing.

Collaborative efforts involving bridging the conventional breeding and newer genomic methods, including use of targeting-induced local lesions in genomes and next generation sequencing assays, would pave the way for developing drought resilient cultivars.

DM performed the HPLC, microarray, bisulfite sequencing assays, analysis and drafted the manuscript.

Identity polymerase chain reaction (PCR) and sequencing assays had confirmed the presence of the 85A insert within the FP9 vector and no wild type FP9 was present.

AL also performed the bisulfite sequencing assays under the supervision of WM and participated in the study design and drafting of the manuscript.

Using ChIP-seq (chromatin immunoprecipitation followed by sequencing) assays, Cieslik et al. showed that EMT is driven by the chromatin-mediated activation of transcription factors [ 41].

Ideally, targeted mtSNPs should retain the maximum level of discrimination power in a single mtDNA test and, therefore, a careful selection of mtSNPs for mini-sequencing assays is a key step in the process.

Using bisulfite PCR-sequencing assays, we observed significantly decreased DNA methylation levels at the exon 10 in HCT116-DKO cells compared with HCT116-WT cells.

Because we found no sequence variation in our products after three independent cycle-sequencing assays, we disregarded the cloning step at NHMUS.