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Deletions of xlnR and clrA were confirmed by PCR using internal and external oligonucleotide primers and by sequencing around the respective loci.
Here however, sequencing around 10% of the gtfB we observed 47 polymorphic sites equivalent to 10.32% of all sites in the 455 bp fragment sequenced.
The structures of the intermediate BACs were verified by restriction enzyme analysis and sequencing around the inserts.
Since the products amplified during the real time PCR were too short for optimal sequencing (around 60 bases long), new reverse primers were designed to extend the amplified sequences over 200 bases long (see table 5).
Furthermore, many candidate gene studies were performed in the post GWAS era, by deep sequencing around GWAS loci.
In total, 3.68 M discrete CpG sites (~13% of all CpGs in the genome) are covered by 50 bp sequencing around "well-spaced" Csp6I sites (Table 1).
Similar(51)
A contig of 220 kb was sequenced around Pdu-Gsx and Pdu-Xlox, and a separate contig of 230 kb was sequenced around Pdu-Cdx.
Col28a1 (NM_001037865.1) was partially amplified and sequenced around exon 16 18 to confirm an in-frame deletion.
Genomic DNA from ten ste−/− mutant and ten wild-type embryos was sequenced around the point mutation.
The PSI-Blast detection rates found here are improved (over 80%), especially compared to the designed SH3 sequences (around 50%).
Moderately methylated sequences (around 30%) were found in the flanking sequences of Kiddo and Toya.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com