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Cycle sequencing and sequencing was performed at the ASGPB sequencing facility of University of Hawaii at Manoa (http://asgpb.mhpcc.hawaii.edu).edu
The sequence of the cloned DNA was determined by dideoxy sequencing, and sequencing ambiguities were resolved by repeated sequencing on both strands.
Genomic DNA extraction, barcode PCR, Illumina sequencing, and sequencing data analysis were performed as described (Sun et al. 2013).
Based on these results we performed sodium bisulfite sequencing and sequencing of the endogenous Rb core promoter in the U87MG cell line.
Sequencing was done using Roche 454 Titanium platform both for shotgun sequencing and sequencing of an 8 kb mate-paired library.
With the advent of rapid DNA sequencing, new species are being discovered by both 16S rRNA gene sequencing and sequencing of metagenomes.
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Cycle sequencing and sequence analyses took place on different thermocyclers and sequencers.
One approach utilizes in vitro selection, high-throughput sequencing, and sequence-specificity landscapes (SEQRS).
Borderline hits were further validated by de novo sequencing and sequence similarity search with MS BLAST.
The authors thank workers in Beijing Genomics Institute for their help in sequencing and sequence annotation.
TM: performed BAC sequencing and sequence assembly.
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