Gene sequencing analyses were performed at Polymorphic DNA Technologies Alamedaa, CA) using an ABI 3730xl DNA sequencer and cycle sequencing, according to the manufacturers protocol.
PCR products were directly sequenced by Ion Torrent PGM sequencer (Life Technologies) after fragmentation, library preparation, purification and cycle sequencing according to manufacturer's instructions.
DNA sequences of new plasmid constructs were confirmed by DNA sequencing according to the chain termination method using the model ABI310 automatic sequencer.
The DNA has been prepared for Illumina sequencing according to the Illumina standard protocol.
GenElute™ PCR clean-up column (Thermo Fisher Scientific, Germany) was used for purification before sending the extracted DNA for sequencing, according to the manufacturer's instructions.
After preparation of the library, an Illumina HiSeq 2000 platform was used for sequencing according to the manufacturer's instructions and paired-end reads were obtained.
Nanopore DNA sequencing method can be divided into bio-nanopore sequencing and solid-state nanopore sequencing according to the nanoporous material [54].
The applications of sequencing technologies on environmental microbiology can be mainly divided into target sequencing, metagenomic sequencing and single-cell genomic sequencing, according to the problems they need to be solved.
The obtained T0 transgenic seedlings were grown in a paddy rice field after screening of tolerant-hygromycin and DNA sequencing according to our described methods (Jiang et al. 2014a, b).
The sheared cDNAs were then prepared for Illumina sequencing according to the manufacturer's protocols.
All clones were verified by DNA sequencing according to their wild-type sequences (See Figure S1).
