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However, the size of the PRN gene renders full gene sequencing a time consuming and moderately costly exercise for allelic typing.
To understand the regulation of the different XTH genes identified through the transcriptome sequencing, a time course study of transcript accumulation of various rose XTH genes was carried out by real-time RT PCR in 0.5 µL L−1 ethylene-treated petal AZs of R. bourboniana and R. hybrida as well as under conditions of natural abscission.
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Alternatively, additional reads can be produced by simply sequencing a sample multiple times.
Individuals with haplotypes that showed unique variation were sequenced a second time to confirm the sequence variation.
In order to confirm a mutation, both strands were sequenced a second time from an independent PCR reaction.
However, when the rabies control plasmid was sequenced a second time, the resulting ORP mismatch rate profile was quite different.
Therefore, this region was re-sequenced a second time and additionally sequenced for all seven clinical isolates; our previous result was confirmed.
Time sequence: a time sequence T = t 1, t 2, …, t n is an order set of values, 1, 2, …, n is the time node with the length of n.
For semiquantification, multiple clones have to be sequenced and all CpGs within the sequence have to be analyzed after sequencing, making bisulfite-sequencing a time- and labor-consuming technique, not suited for fast-track diagnostic testing.
The use of a small flip angle and a gradient for refocusing the magnetization vectors gives this sequence a time advantage.
Complicating matters is that the website limits users to aligning 300 sequences at a time; aligning additional sequences is available on a pay-for-use basis.
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