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Sequencing a number of clones from the test and the reference population of phage-Ab could reveal clones which are differentially represented in the two pools, but clearly, a statistically significant comparison of the two populations would require a very large number of sequences.
RRLs have been extensively used for sequencing a number of individuals in species with a reference genome sequence [ 16, 35].
However, when using more than one enzyme and sequencing a number of different libraries, the necessity for enzyme-specific adapters will increase the total costs.
The 1000 bull genome project [ 15] aims at sequencing a number of key ancestor bulls in the beef and dairy cattle population at medium coverage.
These ancestral polymorphisms were found from sequencing a number of B. taurus breeds and a subset of wild relatives from the Bovinae subfamily without any knowledge about whether they were still segregating in B. taurus.
In addition to sequencing a number of interesting positional candidate genes, we applied whole exome sequencing for two affected individuals in an attempt to unravel the underlying genetic cause within this candidate locus.
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We sequenced a number of PCR products from the NTCs and found the bovine sequences to correspond to European cattle.
We sequenced a number of PCR-products obtained directly from the colony screening o TAR clones using both forward and reverse DBL1alpha -primers.
We also sequenced a number of SNV dense regions with conventional Sanger sequencing.
The grouse study used genomic information from chicken and sequenced a number of candidate genes with Sanger sequencing.
All fragments were double-strand sequenced a number of times, and genetic variations found were checked twice.
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