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The paired-end sequencing (2 × 100 cycles) was performed on HiSeq2000 sequencers (Illumina).
The paired-end sequencing (2 × 100 bp) was performed on an Illumina Hiseq2000 sequencer at the LC Biotech, Hangzhou, China.
Paired-end sequencing (2 × 250 bp) was carried out on an Illumina MiSeq sequencer (Fasteris, Switzerland).
The paired-end sequencing (2 × 251 base pairs) was performed on an Illumina Mi-Seq platform at Scigenome India Pvt Ltd ,Cochin, India.
Paired-end sequencing (2 × 100 bp) was carried out using the Illumina HiSeq X Ten platform (Illumina, San Diego CA, USA) at the Beijing Ori-Gene Science and Technology Co., Ltd.
The first type showed 99.65% similarity to the sequence obtained by direct Sanger sequencing (2 nucleotide changes).
Similar(26)
> -wrap-foot> *Descrined in adult tumor samples To provide a comprehensive landscape of somatic genomic alterations (termed mutational signatures) in cancer genomes, numerous cancers have been profiled by DNA sequencing [ 2, 34, 45].
We recently characterized microbiomes of first-catch urine specimens from adult men using 16S rRNA allele sequencing [2].
Disease-causing mutations in a human cancer patient have also recently be reported through whole-genome sequencing [2], illustrating the rising importance of this experimental strategy.
More SNPs are found as expected in the non coding 3'UTR sequences with 1 SNP every 49 bp but still lower than what was observed with SANGER sequencing [2] of 1SNP every 31 bp for non-coding regions.
An exhaustive molecular analysis is performed using 1° Next generation sequencing, 2° Reverse phase protein arrays and 3° Immuno-histochemistry.
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