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A unique barcode was incorporated into each library during library preparation, and then all libraries were pooled for single-end sequencing (1 × 50 bp) using an Illumina GA-II sequencer.
The promoter of the VILIP-1 expressing lung cancer cell line NCI-H520, was found to be minimally methylated by bisulfite sequencing (1 clone out of eight was found to be methylated) (Figure 3B and 3C).
After SM env sequencing, 1 of 17 sequences from the HIV-1 infected participant was found to be of subtype A and fell in a monophyletic cluster with the seroconverter's sequences.
The infection was confirmed to the molecular level with PCR and gene sequencing (1 ).
Currently, determination of inserted basic amino acids within the hemagglutinin cleavage site of HPAI relies on nucleotide sequencing (1 – 3 ).
Two strains of Rift Valley fever virus (RVFV) were isolated and identified by using indirect immunofluorescence, reverse transcriptase polymerase chain reaction, and sequencing (1, 2 ).
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The technology of next-generation sequencing [1] with its possibility to sequence genomes in an accelerating pace pushed the door open to a new set of targets approachable by existing and future drugs.
First, the H37Rv strain used for the primary sequencing [1] had probably accumulated various mutations during repeated in vitro passages.
There is currently an intense effort to improve the throughput as well as reducing the cost of DNA sequencing [1], [2].
F3 generation epigenetic changes in DNA methylation induced by vinclozolin were previously assessed with the use of methylation-sensitive restriction enzyme digestion and bisulfite sequencing [1].
Paleogenomics, the study of genomes from extinct organisms, is an emerging scientific field that has been fuelled by recently developed technologies in high-throughput DNA sequencing [1], [2].
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