Of the 521 individuals for which both Illumina and Sanger sequences were produced, 429 (82%) produced Sanger and Illumina sequences with no sequence difference.
After discarding sequences with no significant BLAST result, the remaining sequences were trimmed from the 3′ end so that all reads were now 19nt in length.
A previously optimized assay was cloned and the primer and probe sites were mutated to produce novel sequences with no known homology to published sequence data.
Specificity is ensured by the selection of sequences with no homology in BLAST, as well as retention time deviation check, and ion ratio monitoring.
Even beyond these minor technical difficulties, there are problems: an opaque user interface, poorly explained mechanics, and tiresome battle sequences with no discernable connection to the Pokémon games millions of fans have come to know and love.
In a magisterial new work, "The Evolution of Childhood," Melvin Konner, an anthropologist at Emory University, defines play as "inefficient, partly repetitive movements in varied sequences with no apparent purpose".
The reference (zebrafish) genome was split into 21 Mb sequences with 10-kb overlap, while the percomorph fish genomes (H. comes, stickleback, fugu, medaka and Nile tilapia) were split into 10 Mb sequences with no overlap.
As a kid, I used to love thinking of sequences with no dialogues and trying to put the music I was listening to, to sync up to them.
Let be a subset of consisting of sequences with no more than leading zeros.
However, they may not provide accurate results for sequences with no or small object motions.
Sequences with no or several positions on the genomes were excluded.
