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Measurements of the fluorescence anisotropy change on DNA binding indicated that apaMyoD bound to E-box-containing DNA sequences with enhanced affinity relative to MyoD-bHLH.
Spatial coverage in 3D EPI was increased by a factor of 4.5 compared to 3D GRE, while also the SNR was increased by a factor of 2. Image contrast for both magnitude and phase between gray and white matter was similar for both sequences, with enhanced conspicuity of anatomic details in the 3D EPI images due to the increased SNR.
There were 73 genes and EST sequences with enhanced gene expression in all the 5 breast and 3 prostate cancer cell lines when compared with the corresponding normal epithelial cells.
RNAexinv focused on the design of sequences with enhanced thermodynamical and mutational robustness (Avihoo et al., 2011), while Frnakenstein enables to design RNA with multiple target structures (Lyngsø et al., 2012).
In the case of the Pin1 WW domain, the answer is clear: replacement of a six-residue loop that is essential for function with shorter sequences with enhanced propensity to form a β turn increases the folding rate by almost an order of magnitude [ 51].
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We generated a full Wild guinea pig (Cavia aperea) genome sequence with enhanced coverage of methylated regions, benefiting from the available sequence of the domesticated relative Cavia porcellus.
More recently, bench-top high-throughput sequencing instruments such as the Roche/454 GS Junior (Roche, Basel, Switzerland), Illumina MiSeq and Ion Torrent PGM (Life Technologies) have been released, which are capable of sequencing several megabase to gigabase pairs in a single lane-sequencing run with enhanced speed and cost performance.
Magnetic resonance imaging (MRI) has been applied in the study of atherosclerotic disease in different ways: with high-resolution sequences, with contrast-enhanced techniques and with MR angiography (MRA) with and without contrast injection.
Together with increasingly inexpensive next generation sequencing (NGS) techniques it became feasible to sequence various strains with enhanced temperature tolerance (as well as the parental wild type), to identify genes of interest.
Using a recently developed multi-echo resting-state functional MRI (magnetic resonance imaging) sequence with greatly enhanced signal compared to noise ratios, we map frontal cortical functional projections to the striatum and striatal projections through the direct and indirect basal ganglia circuit.
Here, the further molecular reaction sequence is studied with enhanced sensitivity, using MBMS coupled with resonance-enhanced multiphoton ionization (REMPI).
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