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The left sequences were then used to do the following Bayesian evolutionary analysis.
Sequences were then dereplicated and sorted using the -derep_fulllength and -sortbysize commands.
A total of 30,443 such distinct sequences were then used to assemble a mtDNA sequence1.
PCR target sequences were then confirmed by Mayo Clinic's Advanced Genomic Technology Center DNA Sequencing Core.
The remaining sequences were then pooled with WGS scaffolds together to extend and collapse redundant sequences.
Sequences were then polished with PacBio subreads and Illumina paired-end reads (101.3 Gb) (Supplementary Figure 1b, Supplementary Table 1).
The resulting Ger receptor protein sequences were then clustered by neighbor-joining based on sequence alignment.
Sequences were then used to design more sensitive molecular diagnostic tools.
Sequences were then used for microbial diversity analysis using QIIME.
Sequences were then aligned on the RAP-DB website to determine their specificity.
These 300 candidate sequences were then filtered using the filter cascade shown in Table 4.
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