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The targets sequences were screened by using of standard in silico tools and immunoinformatic web servers.
Individual aptamer sequences were screened on the basis of their binding to the 10 M-types that were used as targets.
All non-redundant trimmed sequences were screened for repeat motifs using the Repeatmasker (http://www.repeatmasker.org/), SSRIT (Simple Sequence Repeat Identification) http://www.gramene.org/db/markers/ssrtool using Perl regular expressions to find perfect SSR within the sequences (Temnykh et al. 2001).
The UniRef protein sequences were screened with these HMMs.
Prior to design, sequences were screened for possible indels in A and T homopolymer runs.
First, the sequences were screened against the NCBInr protein databases using BLASTx.
The cDNA sequences were screened for redundancy by BLAST analysis of each constituent against the entire cDNA dataset.
All sequences were screened for vector contamination using the cross_match program from the Phred/Phrap package [39] against NCBI's UniVec database.
Before use, PCR primer sequences were screened across the human genome using the NCBI Blast program to ensure their specificity for the gene of interest.
Repetitive sequences were screened using RepeatMasker.
The vector sequences were screened with the program CROSS_MATCH.
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