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The remaining rRNA sequences were removed for downstream analyses.
Chimeric sequences were removed by UCHIME (Edgar et al. 2011).
Gaps and ambiguously aligned sequences were removed manually from further analyses.
Redundant sequences were removed, forming a specific repeat database for each species.
The adaptors and low quality sequences were removed using Trimmomatic v0.30 (Bolger et al. 2014).
Raw Tags were filtered by Trimmomatic v0.33 and chimeric sequences were removed by UCHIME v4.2.
When initial viewers complained about the film's length, 2 of the 14 song sequences were removed.
Several similar sequences were removed to create a thriller set in the days before Judgement Day.
In this way, recombinant sequences were removed.
Vector sequences were removed as described above.
Unalignable and poor quality sequences were removed from the dataset.
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