Sentence examples for sequences were quantified from inspiring English sources
Amplified PCR products of all actual cDNAs were sequenced to ensure that the correct mRNA sequences were quantified.
Copy numbers of methylated and unmethylated sequences were quantified based on a separate plasmid standard curve for each.
Clusters exclusively comprising sequences with the same label (for example, BioMarKs sequences or cultured ciliate sequences) were quantified.
Genomic sequences were quantified based RepeatMasker matches to the TIGR Solanum Repeat Database v3.3 sequences and quantitated at the sub-class level [ 70].
DNA sequences were quantified with Bio-Rad CFX96 thermocycler using SsoFast EvaGreen Supermix (Bio-Rad, Hercules, CA, USA) according to the manufacturer's instructions.
The TDRMs evoked during translation sequences were quantified as the standard deviation of the time-dependent response averaged across stimulus repetitions.
The genetic variability among ancient sequences was quantified as both nucleotide diversity and haplotype diversity.
The expression level of 27,889 coding sequences was quantified using the software Cufflink.
The protocol for analysis of sequence variation is described in Additional file 8. Essentially, after preparation of multiple alignments, the variation among sequences was quantified and characterised using phylogenetic estimation.
An alignment between two sequences is quantified using an amino acid substitution matrix and a gap penalty setting, both of which are set as prescribed in Saigo et al. [ 24].
The similarity between two sequences is quantified by a similarity statistic, or similarity score, S. Conventionally, the similarity score is found by a weighted sum of the aligned positions between two sequences, in which the weights are given by a log-odds amino acid scoring matrix, such as the BLOSUM62 matrix [ 73].
