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(A) Four nanobodies having different sequences were purified by immobilized metal affinity chromatography (IMAC) using a His-Select column.
All synthesized sequences were purified by HPLC and then quantified.
Plasmids containing cloned sequences were purified using the QIAprep Spin Miniprep Kit QIAGENN).
Sequences were purified using spin columns and submitted to the Vanderbilt University Shared Sequencing Resource.
The sequences were purified and were used in a BLASTn against the Phytophthora database (http://www.phytophthoradb.org/).
Sequences were purified by 70%isopropanol/70%% ethanol precipitation and analyzed with an ABI PRISM® 3730 sequencer (Applied Biosystems).
Similar(49)
The 800bp fragment including the scFv sequences was purified and cloned into the corresponding sites of the pCB5- G2W5 4 pCB5- G2W5 4
A DNA fragment (MluI-SalI) containing the miR-375 promoter and the EGFP ORF (devoid of vector sequences) was purified and microinjected to fertilized mouse oocytes.
In both cases, plasmids possessing the expected sequence were purified using the QIAprep spin miniprep kit (Qiagen #27106) from 3 5 mL LB cultures grown for 16 20 h at 37°C.
PCR products for sequencing were purified using silica spin columns.
PCR products for sequencing were purified using a Gel Extraction Kit (Qiagen, MD, USA).
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