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Furthermore, a clonal analysis of the dispersion of EGFP expression revealed that the IFN-SAR/5′HS4 insulator dyad was particularly effective in reducing the variability of transgene expression when both sequences were placed in opposite orientations within the retroviral backbone.
Split screen sequences were placed towards the end more than the rest of the episode to show the audience there is a bomb at the hotel with several police officers outside the building and Lucas and Ros looking around the hotel for Lawrence and Mudasser.
The endophyte sequences were placed in both Sebacinales subgroups A and B, however they were not evenly distributed across these two groups, as most endophytic sequences were placed in group B (Fig. 1).
A low number of the total sequences were placed in non-trivial assemblies (greater than 2 3 sequences assembling).
Initially, the three independent siRNA-encoding sequences were placed within the lhRNA duplex such that they were spaced at 23 bp intervals.
Thus, a TAA terminator codon was removed and the GFP and ICP0 coding sequences were placed in the same open-reading frame.
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Consensus mature sequences are placed at the top of the box, where only perfectly aligned sequences are capitalized.
This involves a process called multiple sequence alignment in which regions that are conserved among related sequences are placed in vertical alignment, and intervening length-variable regions are modified through the insertion of "gaps".
If miRT sequences are placed in a small single-stranded positive-sense RNA genome such as picornaviruses, they become part of the single viral mRNA [8].
Briefly, the enhanced yellow fluorescent protein coding region (EYFP), flanked with splice acceptor and donor sequences, was placed on a retroviral vector (pBabeAE) and integrated into the genome.
With exception of the TE domain set which has three PKSDB sequences that are located in non-PKS I branches (see Methods S5) all sequences are placed as expected in PKS I branches.
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